Wildlands established its first nature preserve in the Eastern Sierra Nevada back in 2015. This initial acquisition protected 150 acres of land at the confluence of the West Walker and LIttle Walker River in northern Mono County from being subdivided and developed into multiple seasonal vacation homes. In August 2023, The Wildlands Conservancy expanded this property by acquiring the neighboring 2,333-acre Bently Family Junction Ranch, expanding Two Rivers Preserve to 2,483 acres.